SBL 1023 TECHNIQUE IN BIOLOGY AND BIOCHEMISTRY LABORATORY

LABORATORY 4: PROTEIN ANALYSIS OBJECTIVE 1.To determine protein concentration in various type of protein content 2.To determine protein concentration using two types protein assays that are Biuret assay and Lowry assay. I NTRODUCTION There are many ways to measure protein concentration; one of the common protocol is by measuring the absorbance of a coloured product formed by the protein and an organic molecule. Different methods can yield somewhat different results for the same protein as there is no absolute photometric protein concentration assay. All methods have advantages and disadvantages and a few aspects will be taken into consideration: specificity, sensitivity, the measurable range of concentration, the accuracy, the nature of the protein to be examined, the presence of materials interfering with the measurement, and the time required for the measurement. Biuret protein assay use biuret reagent that detect the presence of peptide bonds. When th...

1.0         INTRODUCTION             Lipids are classified as organic compounds that are soluble (dissolvable) in organic            solvents, but only sparingly soluble in water. Lipids are biologically important for making barriers (membranes of animal cells), which control the flow of water and other materials into a cell. There are several classes of lipids, including fatty acids, waxes, triacyglycerols (fats and oils), phospholipids and steroids. The fatty acids are usually not free in nature, but are components of triacyglycerols, waxes, and phospholipids Lipids are major components of cell membranes , and are responsible for most of the permeability filter functions of membranes. Membranes act as barriers to separate compartments within eukaryotic cells, and to separate all cells   from their surroundings. 1.1       ...