SBL 1023 TECHNIQUE IN BIOLOGY AND BIOCHEMISTRY LABORATORY

4.0 Introduction Water microbiology is concerned with the microorganisms that live in water, or can be transported from one habitat to another by water. Water can support the growth of many types of microorganisms. This can be advantageous. For example, the chemical activities of certain strains of yeasts provide us with beer and bread. As well, the growth of some bacteria in contaminated water can help digest the poisons from the water. Because of that, we have decided to take a few samples of water source to investigate the quantity of microorganism in each of these water sample. 4.1 Objectives 1.        To investigate the presence of microorganism in water 2.        To determine which of those water samples contain the most and the least microorganism 3.        To know which one of those water samples is the safest to be consumed 4.2 Materials and Apparatus 1.                    Water sample from the drain, distilled water, purified water and boiled water.

Part 1: Aseptic Technique 1.0      OBJECTIVE            1)  To produce isolated colonies of an organism on an agar plate and useful when we need to seperate organisms in a mixed cultures 2)  To identify bacteria are only valid when performed on pure cultures. 1.1     INTRODUCTION Bacteria are everywhere, and some  are good for us  while others are harmful. Bacteria, viruses, and other microorganisms that cause disease are called pathogens. To protect patients from harmful bacteria and other pathogens during medical procedures, healthcare providers use aseptic technique. An acid/base titration can be monitored with an indicator or with a pH meter. In either case, the goal is to determine the equivalence point of the titration. This is the point at which enough titrant has been added to the analyte to just exactly neutralize the analyte. In this experiment, knowledge of the equivalence point will be used to obtain information about the acid dissociation con

INTRODUCTION As we know, plant carried out photosynthesis and they used two pigments which can be categorize by primary pigments and accessory pigments. A pigment is a molecule that absorbs light. The primary pigments are the chlorophyll, which are called as chlorophyll a and chlorophyll b. The chlorophyll is the green pigments that absorb lights strongly in the blue and red but poorly in the green portions because of electromagnetic spectrum. Hence, the green portion of the solar spectrum is actually reflected, not absorbed. Plant pigment can be identify through separation and isolation of the pigments using a thin layer of paper chromatography. Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries