MICROBIOLOGY: PART 4 (STUDENT PROJECT)

-

4.0 Introduction
Water microbiology is concerned with the microorganisms that live in water, or can be transported from one habitat to another by water. Water can support the growth of many types of microorganisms. This can be advantageous. For example, the chemical activities of certain strains of yeasts provide us with beer and bread. As well, the growth of some bacteria in contaminated water can help digest the poisons from the water. Because of that, we have decided to take a few samples of water source to investigate the quantity of microorganism in each of these water sample.


4.1 Objectives
1.       To investigate the presence of microorganism in water
2.       To determine which of those water samples contain the most and the least microorganism
3.       To know which one of those water samples is the safest to be consumed


4.2 Materials and Apparatus
1.                   Water sample from the drain, distilled water, purified water and boiled water.
2.                   Micropipette
3.                   4 universal bottles
4.                   4 agar plates
5.                   Hockey stick (cell spreader)
6.                   Incubator
7.                   Parafilm


4.2 Methodology
1.       Autoclave all the apparatus that we will use.
2.       Take 10ml of sample water from the drain using a micropipette.
3.       Put the sample water inside the universal bottle. (Make sure you close the lid as soon as you transfer the water in it)
4.       Prepare an agar plate.
5.       Transfer the sample water on the agar plate.
6.       Spread the water on the agar using hockey stick (cell spreader) that has been sterilize.
7.       Seal the plate with parafilm.
8.       Repeat step 2 till 7 using distilled water, purified water and boiled water.
9.       Invert the plates and incubate at 37oC for 24 hour





4.3 Results




4.4 Discussion
What we have discussed is there are presence of microorganism in the sample water that we have investigated. The drain water sample contained the biggest amount of microorganism while purified water contained the smallest amount of microorganism after 24 hours. The safest sample water to be consumed is the purified water while the drain water is extremely dangerous to be consumed.


4.5 Conclusion
       What we can conclude from this experiment is water is one of those mediums that microorganism live in. We also concluded that we should drink purified water because it’s the safest source of water. Second safest water source is distilled water and then boiled water. And the most dangerous source of water is drain water.So we should not consumed the drain water because it might gives us dangerous diseases caused by bacterias.


Comments

Post a Comment

Popular posts from this blog

LAB 3 : TITRATION

-
Image
Title : Titration Introduction      Titration is the slow addition of one solution of a known concentration that called as titrant to a known volume of another solution of unknown solution until the reactions reaches neutralization. Which is indicated by a color change. While acid - base titration are usually used to find the amount of a known acidic or basic substance through acid base reactions. The analyte that is titrand is the solution with an unknown molarity. The reagent is the solution with known molarity that will react with the analyte.       Strong acid like hydrochloric acid has a concentration that normally around 0 to 1. And it can completely dissociate in water easily. While the weak acid such as acetic acid cannot dissociate completely in water. pH is the measure of the concentration of hydrogen ions in a solution. So the lower the pH the higher the concentration of hydrogen ions in the solution.   Objectives A) To observe ...
Read more

LAB 6 : MICROBIOLOGY

-
Image
Part 1: Aseptic Technique 1.0      OBJECTIVE            1)  To produce isolated colonies of an organism on an agar plate and useful when we need to seperate organisms in a mixed cultures 2)  To identify bacteria are only valid when performed on pure cultures. 1.1     INTRODUCTION Bacteria are everywhere, and some  are good for us  while others are harmful. Bacteria, viruses, and other microorganisms that cause disease are called pathogens. To protect patients from harmful bacteria and other pathogens during medical procedures, healthcare providers use aseptic technique. An acid/base titration can be monitored with an indicator or with a pH meter. In either case, the goal is to determine the equivalence point of the titration. This is the point at which enough titrant has been added to the analyte to just exactly neutralize the analyte. In this experiment, knowled...
Read more

MEMBER'S JOTTERS

-
1 ) NASRIAH BINTI JUNUS E20172019172 https://drive.google.com/open?id=1RQ-UHje3_Sskfd5AB5gj38wf-zlxhFlo 2) NUR SHAFIRAH FAMEI BT MOHD KHOMEINI E20172019167 https://drive.google.com/open?id=10CUHNO9ysFC65Aivr0SJObtByOrSPqPq 3) NURUL FARADIANA BINTI ADNAN E20172019185 https://drive.google.com/open?id=1XwQR1vuMVBpQMih4Nb7Goav3tvCBOBiy 4) MARSHA FATIHAH BINTI OMAR https://drive.google.com/open?id=1v5y4b_Ks5aJbAy2JlmZGPegeDps-O7TM 5) NURUL NAJWA BINTI MOHD SHUKOR E20172019174 https://drive.google.com/open?id=1xlIKCUl1tpgzDgAJ2TLPVxthnrJFgwEp
Read more